Part:BBa_K4695011

estJ6

Coding sequence of family IV esterase (EstJ6). This enzyme derived from soil metagenomic library is capable of catalyzing di-ester bond hydrolysis.

Design Notes

FLANKED 5' fusion site: AATG, 3' fusion site: AGGT.
Source sequence was optimized and checked for BsaI and SapI restriction sites using: http://genomes.urv.es/OPTIMIZER/

This part has a START codon at the 5' end of the sequence and no STOP codon at the 3' end because it was designed as a part of a fusion protein with interchangable domains. If you want to express only this coding sequence, please add the STOP codon in your design.

Source

Soil metagenomic library
GenBank: https://www.ncbi.nlm.nih.gov/nuccore/MK037455.1/
Article: https://doi.org/10.1016/j.ecoenv.2019.110148

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 16
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 16
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 16
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 16
  Illegal NgoMIV site found at 498
  
• 1000
  COMPATIBLE WITH RFC[1000]